簡要描述：RHB-Basa 神經干細胞培養基,NS定向分化
Cellartis RHB-A和RHB-Basal是兩款無血清、成分*確定的培養基，用于人類或小鼠神經干細胞（NS）的定向分化獲取、維持培養、擴增、誘導分化成神經細胞。

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英文名稱：Cellartis RHB-Basa Stem Cell Culture Media，Cellartis RHB-A Stem Cell Culture Media

中文名稱：Cellartis RHB-Basa神經干細胞培養基，Cellartis RHB-A神經干細胞培養基  

RHB-A和RHB-Basal是兩款無血清、成分*確定的培養基，用于人類或小鼠神經干細胞（NS）的定向分化獲取、維持培養、擴增、誘導分化成神經細胞等用途。RHB-A可用來培養擴增貼壁的人或小鼠的神經干細胞。在RHB-A中培養的神經干細胞保持著穩定的神經干細胞分化發育成為成熟的神經細胞的能力 (1-3) 。另外，RHB-A還用來定向分化小鼠胚胎干細胞(mouse ES)至神經前體細胞 (neural precursors) (4-6)。

RHB‐Basal不包含生長因子或是神經細胞相關的添加物。因此，可以RHB‐Basal為基礎，通過添加客戶認為需要的添加物來開發適合客戶的特殊類型細胞的培養基。

RHB-A and RHB-Basal is a fully defined, serum-free cell culture medium for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells. This optimized medium ensures the long-term stability of NS cell differentiation capacity (1) and can be used to support differentiation of human and mouse NS cells into functional neurons (1–3) and of mouse ES cells into neural precursors (4–6).

RHB‐Basal does not contain any growth factors or neuronal supplements.  Therefore, this media can be tailored to the specific requirements of your cell type by the addition of supplements.

產品特點：(NS細胞：神經干細胞）

RHB-A是一種專有的成分確定的無血清培養基，用于維持人類和小鼠貼壁NS細胞群培養

RHB‐Basal不含動物成分，不含神經元添加劑；培養基可以通過補充添加劑來定制

RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells

RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements

產品應用(ES：胚胎干細胞）

從ES細胞和胎兒和成人組織中分離培養小鼠和人類NS細胞

小鼠和人類貼壁NS細胞的維持和增殖

小鼠和人類NS細胞分化為功能性神經元

小鼠ES細胞向神經元前體的分化

有關使用的其他示例，請參閱數據表

Derivation of mouse and human NS cells from ES cells and fetal and adult tissues

Maintenance and propagation of adherent mouse and human NS cells

Differentiation of mouse and human NS cells into functional neurons

Differentiation of mouse ES cells to neuronal precursors

Refer to the Data Sheet for additional examples of use

組份：

500 ml medium

參考文獻：

1. Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, and Smith A. (2008) Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Molecular and Cellular Neuroscience. 38: 245-258.

2.Conti L, Pollard SM, Gorba T, Reitano E, Toselli M, Biella G, Sun Y, Sanzone S, Ying QL, Cattaneo E, and Smith A. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology. 3(9): e283.

3.Pollard SM, Conti L, Sun Y, Goffredo D, and Smith A. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex. 16: 112-120.

4.Ying QL, Stavridis M, Griffiths D, Li M, and Smith A. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent mono-culture. Nature Biotechnology. 21: 183-186.

5.Pollard SM, Wallbank R, Tomlinson S, Grotewold L, and Smith A. (2008) Fibro-blast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience. 38: 393-403.

6.Pollard SM, Yoshikawi K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire J, Smith A, and Dirks P. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell. 4: 568-580.

7.Diogo MM, Henrique D, and Cabral JM. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Bio-technology and Applied Biochemistry. 49: 105-112.

8.Abranches E, Silva M, Pradier L, Schulz H, Hummel O, Henrique D, and Bek-man E. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE. 4(7): e6286.

9.Hansen DV, Lui JH, Parker PRL, and Kriegstein AR. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature. 464(7288): 554-561.

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