簡要描述：BioLife CryoStor CS5 細(xì)胞DMSO凍存液現(xiàn)貨：
CryoStor CS5細(xì)胞凍存液特點(diǎn)
用于細(xì)胞和組織的高級(jí)生物保存液
預(yù)混DMSO
無血清、無蛋白
USP級(jí)高質(zhì)量成分、cGMP級(jí)生產(chǎn)、FDA master文件
滅菌、內(nèi)毒素和細(xì)胞功能檢測(cè)

BioLife Solutions GMP級(jí)CryoStor CS5 Freeze Media細(xì)胞DMSO凍存液

產(chǎn)品英文名 BioLife Solutions CryoStor CS5 Freeze Media

產(chǎn)品中文名 BioLife Solutions CryoStor CS5細(xì)胞DMSO凍存液

產(chǎn)品介紹  BioLife Solutions CryoStor®是BioLife公司特別優(yōu)化的細(xì)胞凍存液，在極低溫度 (-70ºC to -196ºC)下準(zhǔn)備和保存細(xì)胞。預(yù)混DMSO，為細(xì)胞和組織的冷凍、儲(chǔ)存和解凍過程提供安全的保護(hù)環(huán)境。CryoStor®通過調(diào)控凍存過程的分子生物學(xué)反應(yīng)，無血清、蛋白或具有高細(xì)胞毒性的試劑，就能增強(qiáng)細(xì)胞活力和功能。

產(chǎn)品訂購信息

CryoStor CS5細(xì)胞凍存液特點(diǎn)

用于細(xì)胞和組織的高級(jí)生物保存液

預(yù)混DMSO

無血清、無蛋白

USP級(jí)高質(zhì)量成分、cGMP級(jí)生產(chǎn)、FDA master文件

滅菌、內(nèi)毒素和細(xì)胞功能檢測(cè)

DMSO無血清細(xì)胞凍存液適用細(xì)胞

多種類型細(xì)胞，包括如肝細(xì)胞之類的敏感細(xì)胞

BioLife Solutions CryoStor CS5 細(xì)胞DMSO凍存液cGMP級(jí)生產(chǎn)

MATERIALS ARE MANUFACTURED UNDER cGMP

CryoStor® Usage and Cryopreservation Protocol使用與凍存方法

細(xì)胞準(zhǔn)備

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.

加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor®

a. Cell concentrations: 0.5-10 x 106cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor® - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

儲(chǔ)存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

細(xì)胞復(fù)蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.Passive thaw is not recommended.

9) Dilute cell/CryoStor® mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

BioLife Solutions CryoStor CS5/CS10 Freeze Media 細(xì)胞凍存液

CS5和CS10主要區(qū)別是規(guī)格包裝不同。

BioLife Solutions CryoStor Freeze Media GMP級(jí)細(xì)胞凍存液現(xiàn)貨供應(yīng)歡迎您咨詢華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：1500 1904 520。紅榮微再-客服:  經(jīng)銷商專員