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    首頁 > 技術文章 > 全血RNA管和RNA保護液

    全血RNA管和RNA保護液

    更新時間:2017-11-30瀏覽:4744次

    區別

     

    PAXgene® Blood RNA Tube 全血RNA管

    適用:血液樣本

     

    Ambion® RNAlater™ Stabilization SolutionRNA 保護液、固定液

    適用:組織樣品或液氮冷凍樣品

     

    PAXgene® Blood RNA Tube 全血RNA管

     

     

     

    產品英文名 PAXgene® Blood RNA Tube

    產品中文名 PAXgene® 全血RNA管

    產品介紹  PAXgene® 全血RNA管能穩定血細胞內的RNA水平,提供一份zui接近人體內狀態的RNA標本,延長血液標本體外放置的時間,利于批量采集,細胞轉錄水平保真性好,可用于與血細胞RNA相關的RT-PCR檢測,人類疾病基因轉錄譜的研究及其他RNA轉錄相關的檢測。特殊RNA穩定劑、zui小化體外血RNA降解或基因誘導,延長標本體外放置時間,全血RNA原管室溫保存3~5天,凍存條件下儲存8年。

     

    產品訂購信息

    品牌

    貨號

    產品描述

    包裝

    BD

    762165

    2.5mL全血RNA管,6.9mL添加劑,紅色安全蓋

    100/箱

     

    詳細說明

    Tube Material            Plastic

    Tube Size              16x100 mm

    Draw Volume             2.5 mL

    Closure Type            BD Hemogard™

    Closure Color            Red

    Label                Paper

    Cell Separator           No

    Additive              6.9 mL of Proprietary RNA stabilization additive

    Sterilization               Radiation, 10-6 SAL, ISO11137-1

    Regulatory Status            IVD

     

    Intended Use

    The PAXgene® Blood RNA System consists of a blood collection tube (PAXgene® Blood RNA Tube) and nucleic acid purification kit (PAXgene® Blood RNA Kit). It is intended for the collection, storage, and transport of blood and stabilization of intracellular RNA in a closed tube and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.

    Performance characteristics for the PAXgene® Blood RNA System have only been established with FOS and IL1B gene transcripts. The user is responsible for establishing appropriate PAXgene® Blood RNA System performance haracteristics for other target transcripts.

     

    Warnings

    1. Contents of this tube are irritating to skin.

    • After inhalation, supply fresh air: consult doctor in case of complaints.

    • After skin contact, immediay wash with water and soap, and rinse thoroughly.

    • After eye contact, rinse opened eye for fifteen minutes under running water, then consult a doctor.

    • After swallowing, immediay call a doctor.

    A blood collection set must be used with the PAXgene® Blood RNA Tube. See Ordering Information.

     

    Limitations

    1. Under-filling of the PAXgene® Blood RNA Tubes will result in an incorrect blood-to-additive

    ratio and may lead to incorrect analytic results or poor product performance.

    2. The PAXgene® Blood RNA System is not suitable for the collection and purification of viral

    RNA.

    3. Blood samples obtained with the PAXgene® Blood RNA Tube must be prepared only with the PAXgene® Blood RNA Kit. For catalog numbers, see Ordering Information.

    4. The quantity of blood drawn should be 2.5mL per PAXgene® Blood RNA Tube, but this volume might vary with altitude, ambient temperature, barometric pressure, tube age, venous pressure, and filling technique.

    5. The PAXgene® Blood RNA Kit is intended for purification of intracellular RNA from human whole blood with leukocytes counts between 4.8 x 106- 1.1 x 107leukocytes/mL.

     

    Precautions

    1. Practice universal precautions. Use gloves, gowns, eye protection, other personal protective equipment, and engineering controls to protect from blood splatter, blood leakage, and potential exposure to bloodborne pathogens.

    2. Handle all biologic samples and blood collection sets "sharps" according to the policies and procedures of your facility. Obtain appropriate medical attention in the event of any exposure to biologic samples (for example,through a puncture injury), since they may transmit viral hepatitis, HIV (AIDS), or other infectious diseases.Utilize any built-in needle protector, if the blood collection set provides one. PreAnalytiX does not recommend reshielding used needles. However, the policies and procedures of your facility may differ and must always be followed.

    3. Discard all blood collection tubes in biohazard containers approved for their disposal.

    4. Do not re-use the PAXgene® Blood RNA Tubes.

    5. Do not use the PAXgene® Blood RNA Tubes after the expiration date printed on the tube label.

    6. Since the PAXgene® Blood RNA Tube contains a chemical additive, a blood collection set must be used for blood collection to prevent possible backflow from the tube. See Specimen Collection and Preparation for Analysis section.

    7. Excessive centrifugation speed (over 10,000 RCF) may cause the PAXgene® Blood RNA Tube breakage, exposure to blood and possible injury.

    8. Do not transfer a sample from a syringe to a tube.

     

    Storage

    Store the unused PAXgene® Blood RNA Tubes at 18ºC to 25ºC. Limited excursion temperatures up to 40ºC are permitted.

     

    762165 現貨歡迎您致電 華雅再生醫學旗艦公司:紅榮微再(上海)生物工程技術有限公司  :1500 1904 520。紅榮微再-客服: 2395557778  經銷商專員

    Ambion® RNAlater™ Stabilization Solution RNA保護液

     

     

     

     

     

    詳細介紹

    產品英文名 Ambion® RNAlater™ Stabilization Solution

    產品中文名 Ambion® RNAlater™ RNA 固定液

    產品介紹  Ambion® RNAlater™ Stabilization Solution: RNA固定液為水溶性、無組織毒性的保存試劑,可快速滲透到組織中,固定并保護細胞RNA。RNAlater® 固定并保護未破壞的未冷凍組織樣品中的細胞RNA,無需為后續處理而快速處理組織樣品或液氮冷凍樣品。組織塊可獲得并浸入RNAlater® 中進行保存,不影響后續RNA分離所獲得的RNA數量和質量。RNAlater® 可加到細胞團甚至培養基中的細胞中,然后樣品即可冷凍或不冷凍保存。

     

    Ambion® RNAlater™ Stabilization Solution訂購信息

     

    品牌

    貨號

    產品描述

    包裝

    單價

    Thermo

    AM7020

    Ambion® RNAlater™ Stabilization Solution

    RNA 保護液、固定液

    100 mL

    1422元

    Thermo

    AM7021

    Ambion® RNAlater™ Stabilization Solution

    RNA 保護液、固定液

    500 mL

    4444元

     

     

    RNAlater™ Stabilization Solution RNA 保護液特點

    • 一種試劑即可迅速滅活RNA酶,穩定組織或細胞中的RNA

    • 無需液氮冷凍樣品或將樣品快速轉入實驗室冰箱中

    • 多數組織樣品無需冷凍和研磨

    • “野外”組織采集的理想選擇

    • RNA在 37°C 下可穩定1天, 25°C 下1周, 4°C 下1個月, -20°C 下無限期。

    • 與包括大多數RNA分離試劑盒在內的多數RNA分離程序相兼容

     

    RNA保護液上下游產品

     

    品牌

    貨號

    產品描述

    包裝

    Thermo

    4444436

    TaqMan Fast Virus 1-Step Master Mix 酶

    10mL/瓶

    Thermo

    AM9920

    DEPC-Treated Water DEPC處理水

    500mL/瓶

    Thermo

    15567027

    UltraPure 1M pH7.5 Tris-HCI Buffer緩沖液

    1 L/瓶

    Thermo

    AM9262

    EDTA (0.5 M), pH 8.0 溶液

    1000mL/瓶

    Thermo

    AM2222

    Ambion DNase I (RNase-free) 酶

    2000U/支

    Thermo

    12574026

    SuperScriptIII One-Step RT-PCR含Taq DNA 酶

    100次/盒

    QIAGEN

    57704

    QIAamp MinElute Virus Spin Kit(50)試劑盒

    50次/盒

    Promega

    P2077

    T7 RNA Polymerase 聚合酶

    5000U/支

    Corning

    21-031-CVR

    DPBS(1x)緩沖液(不含鈣鎂)

    500mL/瓶

    NEB

    M0363L

    T5 核酸外切酶

    5000U/組

     

    歡迎您致電 華雅再生醫學旗艦公司:紅榮微再(上海)生物工程技術有限公司  :1500 1904 520。紅榮微再-客服: 2395557778  經銷商專員

     

     

     

     

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